In vitro cloning of female and male Carica papaya through tips of shoots and inflorescences

Abstract

for 7 days; Step 3- Roots at the cut end of about 95% shoots were visible, after about 10 days, in the same medium as used in Step 1, but having supplements of 3 vitamins, 2 amino acids and 0.25 mg l −1 IAA, while sucrose was removed; and Step 4- The just rooted shoots when finally transferred to the semi-sterilized moist Soilrite contained in culture tubes, formed healthy roots without intervening callusing, while the shoots also remained healthy. Such plantlets when ultimately transplanted along with Soilrite plugs to the potted soil showed about 80% transplant success. In vitro-raised plants appeared normal and fruited under field conditions after about 6 months of ex vitro growth.